Location

Seahorse Facility

This facility houses a real-time metabolic analyser which enables you to measure metabolic data on energy production in live cells

LHS image is schematic illustration of mitochondrial metabolism. RHS of image is a photograph of a Seahorse XFe 24 analyser with PC
Address

Mailbox 6
L6/083
School of Medicine
Ninewells Hospital
Dundee
DD1 9SY

Campus
Ninewells Campus
Group or department

About

The School of Medicine Seahorse Facility is a dedicated laboratory for live cell metabolic analysis. It currently has an Agilent Seahorse XFe24 analyser. In live cells, the Seahorse XFe24 analyser measures both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) which are important indicators of mitochondrial respiration and glycolysis.

Equipment & Facilities

The facility 

  • Agilent Seahorse XFe 24 Analyser
  • HERA Cell 150 CO2 incubator
  • Binder Incubator
  • Class 1 Microbiological Safety Cabinet
  • Water Bath

The Seahorse XFe24 utilises a 24 well plate. Each well on the 24-well plate has 4 drug injection ports, where you can add inhibitors, stimulants, or substrates (these are defined by user). Example assay kits are mitochondrial function, drug-induced mitochondrial toxicity, substrate and palmitate oxidation stress, real time ATP production.

The Seahorse Facility can provide sensor cartridge/ culture plates (FluxPak) for a charge, or you can purchase your own. The facility is booked and charged as a per-half day rate.

A laboratory with different general laboratory equipment on LHS including safety cabinet, gas cylinders, bench top incubator and CO2 incubator, under-bench freezer/fridges. RHS laboratory bench has an Agilent Seahorse Analyser with laboratory bench and stools underneath. The benches are running down either side with the laboratory door at the end.
Enquiries

Dr Gwen Kennedy

+44 (0)1382 383048

[email protected]